초록 close

Pedioccus acidilactici produces bacteriocin,which kills Listeria monocytogenes. The bactericidal mode ofaction of the bacteriocin against L. monocytogenes V7 wasinvestigated by transmission electron microscopy. The bacteriocinwas purified partialy from the cel-free extract using Micro-Cel and cation-exchange chromatography, and the specificactivity was increased 1,791 fold. The bacteriocin (6,40 AU/ml) was inoculated with L. monocytogenes V7 and incubatedfor 0.5 h, 1 h, 3 h, and 6 h. The bacteriocin was found todestroy most of the cel wall and released most of theinclusions in the cells after 6 h of incubation. These resultsto bacterial lysis.