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ditions were studied as a function of cell age. The young and old cells were separated from fresh rabbit blood by using Percoll densitygradients. A novel multi-dimensional microscope was employed to perform real-time, non-invasivein situmeasurements on the mem-brane bending elastic modulus and the cell shape and size. A phase-analysis micro-electrophoresis laser scattering technique was usedto measure the surface charge density. The results show that the membrane Zeta potential of red blood cells is reduced when they become.