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The eect of pH on the mineralization of calcium phosphate regulated by broin is investigated in this paper. Calcium phosphatesform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results dem-onstrated the great inuence of pH on the formation of calcium phosphate, both in the presence and in the absence of broin. Purehydroxyapatite (Ca10(PO4)6(OH)2,HA) can be obtained at relatively high pH (pH 8.0), while brushite (CaHPO4 Æ2H2O, DCPD) isto HA and regulate the morphology and structure of the HA crystals obtained.