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Functionalization of a self-organized poly(amidoamine) (PAMAM) dendrimer monolayer with oligopeptides on a silicon/quartz sub-strate is investigated in this paper. A monolayer of PAMAM dendrimers is formed on a silicon/quartz surface via electrostatic adsorp-tion. The functionalization of the PAMAM monolayer with oligo-tryptophan (Trp) is performed byin situring-opening polymerizationof TrpNspectroscopy (XPS) and UVVis spectroscopy, which conrm that the self-organized PAMAM dendrimer monolayer can be used asan anchoring scaold for the construction of thin oligopeptide lm.