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Cels consistently face stressful conditions, which cause them to modulate a variety of intracellular processes and adapt to these environmental changes via regulation of gene expression. Hyperosmotic and oxidative stresses are significant stresors that induce cellular damage, and finally cell death. In this study, oligonucleotide microarays cells exposed to hyperosmotic or oxidative conditions. In addition, since heat shock protein 70 (HSP70) is one of the most inducible stress proteins and plays pivotal role to protect cels against stressful condition, we performed microarray analysis in HSP70overexpressing cels to identify the genes expressed in a HSP70 dependent manner. Under hyperosmotic or oxidative stress conditions, a variety of genes showed altered expression. Downregulation of protein phosphatase1 beta (P1 beta) and sphingosine1 phosphate phosphatase 1 (SPase1) was HSP70overexpressing cells demonstrated that diverse mRNA species depend on the level of celular HSP70. Genes encoding lysyl oxidase, thrombospondin 1, and procollagen displayed altered expression in all tested conditions. The results of this study wil be useful to construct networks of stress response genes.