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Objectives : In the present study we hypothesized that the change of cytokine profiles can be identified after bee venom treatment and that is associated with modulation of immune-activating cells which have pathogenic role in rheumatoid arthritis. Methods : T cells and monocytes were isolated from human peripheral blood. Isolated monocytes were further induced to differentiate into dendritic cell(DC). Isolated T cells and differentiated DC were activated with phorbol 12-myristate 13-acetate(PMA) and lipopolysaccharide(LPS), respectively. Bee venom(BV) added to each experiment group. Intracytoplasmic stain of T cells and morphologic observation, surface molecule analysis of DC were performed before and after cellular activation. Results : Pro-inflammatory cytokine was reduced whereas anti-inflammatory cytokine was increased with BV treatment in T cells. In DC, morphologic maturation and surface molecule increase by LPS was also inhibited by BV. Conclusions : BV may be an effective treatment in rheumatoid arthritis through its immune-modulating effect.


Objectives : In the present study we hypothesized that the change of cytokine profiles can be identified after bee venom treatment and that is associated with modulation of immune-activating cells which have pathogenic role in rheumatoid arthritis. Methods : T cells and monocytes were isolated from human peripheral blood. Isolated monocytes were further induced to differentiate into dendritic cell(DC). Isolated T cells and differentiated DC were activated with phorbol 12-myristate 13-acetate(PMA) and lipopolysaccharide(LPS), respectively. Bee venom(BV) added to each experiment group. Intracytoplasmic stain of T cells and morphologic observation, surface molecule analysis of DC were performed before and after cellular activation. Results : Pro-inflammatory cytokine was reduced whereas anti-inflammatory cytokine was increased with BV treatment in T cells. In DC, morphologic maturation and surface molecule increase by LPS was also inhibited by BV. Conclusions : BV may be an effective treatment in rheumatoid arthritis through its immune-modulating effect.