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In order to improve the light fastness of a natural colorant berberine, several natural antioxidants such as gallic acid, L-ascorbic acid, and α-tocopherol were applied by aftertreatment method. Even though the increase in light fastness of berberine colorant was not substantial, L-ascorbic acid was the most effective. It was considered that this improvement of the light fastness was due to high antioxidation action of L-ascorbic acid to colorant, which led very highly excited species of the colorant formed by photo-energy to return to the original stable state without being decomposed.