초록 close

In the course of screening program for VHR DS-PTPase (dual-specificity protein tyrosine phosphatase) from natural sources, Gastrodia elata was selected. One compound showing potent inhibitory activity was isolated by the solvent extraction and column chromatography including silica gel, ODS RP-18, Sephadex LH-20, and HPLC. This compound was identified as baicalein by several NMR techniques such as 1H-NMR, 13C-NMR, and DEPT. Baicalein showed selective inhibitory activity against VHR DS-PTPase with IC50=2.4mM, and showed cytotoxicity against several human cancer cell lines with an GI50 of 5.26~12.93mg/mL range, including, melanoma (LOX-IMVI), lung cancer (NCI H23 and A549), colon cancer (HCT 116 and SW 620), prostate cancer (PC-3), and leukemia (MOLT 4F).