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To evaluate the effect of punctal occlusion on tear preservation after third eyelid resection, 10 eyes wereoperated in dogs and observed changes in tear preservaperformed on five healthy dogs with no clinical sings of ocular irritation and no history of dry eye syndrome. TheST was performed for 10 days before surgery, and on the day 0, 1, 3, 5, 7, 9, 11, 13, 17, 21, 25, and 30 afterthird eyelid resection. The mean STT was 17.4±0.15 mm/min(mean±SEM) for 10 days before surgery. And, it was11.5±0.55 m/min for 30 days after surgery in puntal occlusion(PO) group. The mean ST was 17.3±0.32 mm/minfor 10 days before surgery. And, it was more decreased to 6.5±0.60 m/min for 30 days after surgery in controlgroup. The paired t-test was performed. Mean ST after the third eyelid resection was higher in PO group than thatof control throughout experimental period (p<0.05). In summary, the effect of thermal punctal occlusion significantlyincreased on the tear preservation by 28.86±7.96%. These results suggest that application of thermal punctal occulsionin dry eye syndrome will promote tear preservation.