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This study was performed to investigate the reverse effects of atipamezole in green iguana anesthetizedwith intramuscular administration of medetomidine-tiletamine/zolazepam (Zoletil). Heart rate, respiratory rate and bodytemperature were measured. Anesthetic depth was evaluated by righting reflex. In all study groups, heart rate andrespiratory rate significantly decreased at 5 min after anesthetic administration, and gradually increased after atipamezoleadministration. The present study suggested that 50g/kg atipamezole was effective reversal dosage for 100g/kgmedetomidine and 10 mg/kg tiletamine/zolazepam combination anesthesia in green iguanas.