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The study was to evaluate psychological and physiological changes of simulator sickness in the controlled condition of driving a car (1 hr. at speed of 60 km/h) in a graphic simulator. Simulator sickness was measured and analyzed every 5 min using both subjective responses(i.e., Simulator Sickness Questionnaire) and Physiological signals(EEG, HRV, Skin Temperature, GSR). The results showed that there was significant differences in subjective response 10 min after the main experiment. From 10 min after the driving, the level of subjective simulator sickness increased significantly, relative one of the rest condition. There also was significant differences in physiological responses between the rest and the 5 min after from the start of driving: for EEG, and at Fz area increased, while decreased; the averaged R-R interval and skin temperature decreased; LF/HF and GSR increased. The results indicated that simulator sickness was induced by activation of the autonomic nerves and inactivation of the central nerves.