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steroidal saponin, 3-O-α-L-rhamnopyranosyl-(1. 2)-[α-L-rhamnopyranosyl-(1. 3)]-β-D-glucop-yranosylpennogenin (1, spiroconazole A) was isolated from the tubers of Dioscorea bulbifera L. var sativa and1H-and 13C-NMR assignment was completed using HMBC corelation. In addition, four phenolic substances, 2,7-dihydroxy-4-methoxyphenanthrene (2), quercetin (3), quercetin-3-O-β-D-glucopyranoside (4), and quercetin-3-O-β-D-galactopyranoside (5) were also isolated. KeywordsDioscorea bulbifera L. var sativa, steroidal saponin, phenanthrene, flavonoid, HMBC