초록 close

The complete genome sequence of the garlic latent vi-rus (GLV) has been determined. The whole GLV ge-nome consists of 8,353 nucleotides, excluding the 3¢-end poly(A)+ tail, and contains six open-reading frames (ORFs). Putative proteins that were encoded by the reading frames contain the motifs that were conserved in carlavirus-specific RNA replicases, NTP-dependent DNA helicases, two viral membrane-bound proteins, a viral coat protein, and a zinc-finger. Over-all, the GLV genome shows structural features that are common in carlaviruses. An in vitro translation analy-sis revealed that the zinc-finger protein is not pro-duced as a transframe protein with the coat protein by ribosomal frameshifting. A Northern blot analysis showed that GLV-specific probes hybridized to garlic leaf RNA fragments of about 2.6 and 1.5 kb long, in addition to the 8.5 kb whole genome. The two subge-nomic RNAs might be encapsidated into smaller viral particles. In garlic plants, 700 nm long flexuous rod-shaped virus particles were observed in the immu-noelectron microscopy using polyclonal antibodies against the GLV coat proteins.