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A robust and fast DNA chip method was developed in order to detect the various b-lactam antibiotic-resis-tance genes in one slide. These genes included PSE, OXA, FOX, MEN, CMY, TEM, SHV, OXY, and AmpC. b-lactam antibiotic-resistance genes were labeled with a fluorescent nucleotide by a multiplex polymerase chain reaction using a mixture of specific primer sets for each gene. This labeled target was hybridized with a DNA chip that contained the spots of the specific probe DNAs for each b-lactam antibiotic-resistance gene. This technique made it possible to detect the spe-cific resistance gene, even in a single bacterium.