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quantitative analytical method for the content of dioscin in Dioscorea rhizoma using a high performance liquidchromatography (HPLC) system was established for its quality control. After extracting Dioscorea rhizoma flour with ethanol,dioscin was separated through an ODS column with a flow rate of 1.0 ml/min of acetonitril-methanol-0.01 M phosphate bufferwere about 7.45±0.34 min and calibration curve showed good linearity at concentrations from 0.1 to 2.0 mg/ml of dioscin.When 5 commercial Dioscorea rhizoma flours were analyzed by HPLC, the peak for dioscin was well separated from othersin ethanol extract of Dioscorea rhizoma, and the amount of dioscin was in the range from 0.076 to 0.142(w/w)%.Key wordsDioscorea rhizoma, dioscin, HPLC, quantitative determination. ..... Dioscorea . ... .... .. ..