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Twenty primers of 20 mer referred to universal riceprimer (URP) were developed from a repetitive sequenceof rice genome. URP-PCR protocol employedstringent PCR with high annealing temperaturethroughout the thermo-cycling reaction, giving highreproducibility. Under the PCR condition, each singleURP primer produced characteristic fingerprints fromdiverse genomes containing 14 plants, 7 animals and 6microbes, indicating its universal applicability. Thegenerality of URP-PCR was demonstrated by applyingit to 15 cultivars from five rice species, 23 isolates infour Alternaria species producing host-specific toxinson different host plants and 12 bacterial strainsincluding Escherichia coli, Salmonella spp., andBlucella abortus. PCR approach using URP primerswill be useful for studying DNA diversity of mosteukaryotic or prokaryotic genomes, especially at interandintraspecies levels.