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개 난자의 체외성숙과 체외수정을 통해 수정란을 획득하기 위하여 개 난소를 채취하여 난자의 형태, 난소의 채취시기 등이 체외성숙과 체외수정에 미치는 영향을 구명하기 위하여 수행하였다. 1. 난구세포 부착 및 미부착 난자를 48시간 배양했을 때 체외 성숙율은 42.0±3.4%, 24.4±4.1%였다. 난구 세포 부착 난자의 체외 성숙율이 미부착 난자의 체외 성숙율보다 유의하게 높게 나타났다(p>0.05). 2. 난소를 휴지기, 난포기 및 황체기로 구분하여 난자를 회수하여 배양했을 때 체외 성숙율은 각각 35.6%, 50.0%, 31.1%였고 체외 발생율은 각각 2.2%, 20.0% 및 8.9%로서 난구세포 부착 난자가 미부착 난자에 비해 유의한 체외 수정율을 나타냈다. 3. 난구세포 부착 및 미부착 난자를 이용하여 체외수정시켰을 때 체외 수정율과 체외 발생율은 각각 48.0%, 35.6% 및 22.5%, 13.3%로서 난구세포 부착 난자가 미부착 난자에 비해 유의하게 높게 나타났다.


The studies were carried out to investigate the effects of morphology and collection time of oocytes on in vitro maturation and fertilization of canine oocytes. The results were summarized as follows : 1. The in vitro maturation rates of oocytes with or without cumulus cells were 42.0±3.4%, 24.4±4.1%, respectively. The IVM rate of oocytes with cumulus cells was higher than that of oocytes without cumulus cells. The rate of oocytes with cumulus cells was higher than that of denuded oocytes. 2. IVM rate of oocytes recovered from ovaries inactive, follicular and luteal stage was 35.6 %, 50.0%, 31.1%, respectively. In vitro developmental rates to 16 cell stage were 2.2%, 20.0%, 8.9% respectively. In vitro fertilization rate of oocytes with cumulus cells were significantly lower than that of denuded oocytes. 3. In vitro fertilization and developmental rates of oocytes with cumulus cells for 48 hrs was 48.0%, 35.6% and 22.5%, 13.3%, respectively. These rates of oocytes with intact cumulus cells were higher significantly than that of ocytes with denuded cumulus cells.