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From the leaves of Brassica juncea, an radical scavenging isorhamnetin 7-O-glucoside on peroxynitrite and 1,1-diphenyl-2-picrylhydrazyl (DPPH) was isolated and characterized based on the spectroscopic evidence. The compound showed the peroxynitrite and DPPH scavenging activities with IC50 values of 2.07 ± 0.17 and 13.3 mM, respectively. Penicillamine and L-ascorbic acid as positive control exhibited radical scavenging activities with IC50 values of 3.17 ± 0.39 and 12.78 mM, respectively.