초록 close

ABSTRACT Peroxynitrite Scavenging Activity of Samjunghwan Objectives : Peroxynitrite (ONOO-), ․O2- and nitric oxide (NO) are cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer's disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate ONOO- scavenging activities, and that of its precursors, NO and ․O2- of Samjunghwan. Methods : To investigate ONOO-, NO, ․O2- scavenging activities, fluorescent probes, namely 2‘,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Results : Samjunghwan markedly scavenged authentic ONOO-, ․O2- and NO. It also inhibited ONOO- induced by ․O2- and NO which are derived from SIN-1. Furthermore, Samjunghwan inhibited ONOO-, ․O2- and NO generation in LPS-treated ICR mouse kidney postmitochondria. Conclusions : These results suggest that Samjunghwan is an effective ONOO-, ․O2- and NO scavenger, and that this substance has a potential role as an inhibitor of aging process, and in therapy against age-related diseases.


ABSTRACT Peroxynitrite Scavenging Activity of Samjunghwan Objectives : Peroxynitrite (ONOO-), ․O2- and nitric oxide (NO) are cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer's disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate ONOO- scavenging activities, and that of its precursors, NO and ․O2- of Samjunghwan. Methods : To investigate ONOO-, NO, ․O2- scavenging activities, fluorescent probes, namely 2‘,7’-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Results : Samjunghwan markedly scavenged authentic ONOO-, ․O2- and NO. It also inhibited ONOO- induced by ․O2- and NO which are derived from SIN-1. Furthermore, Samjunghwan inhibited ONOO-, ․O2- and NO generation in LPS-treated ICR mouse kidney postmitochondria. Conclusions : These results suggest that Samjunghwan is an effective ONOO-, ․O2- and NO scavenger, and that this substance has a potential role as an inhibitor of aging process, and in therapy against age-related diseases.