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Objectives:It is well known that hexavalent chromium has toxic effect on normal cells. Recently, toxic effect of hexavalent chromium is diminished by the some extracts derived from herbs or plants . But, the toxic or protective mechanism of hexavalent chromium is well unknown. This study was performed to examine the protective effect of poncirin against Na2Cr2O7-induced cytotoxicity on NIH3T3 fibroblasts. Methods:The protective effect of the cytotoxicity induced by Na2Cr2O7 was measured by the cell viability after NIH 3T3 fibroblasts were cultured with or without Na2Cr2O7 for 48 hours. Antitoxic effects of poncirin on the cytotoxicity induced by Na2Cr2O7 were examined by colorimetric assays such as MTT or XTT assay. Results:Na2Cr2O7 decreased cell viability by the decreased absorbance in MTT or XTT assay, but, the poncirin increased cell viability which was decreased by Na2Cr2O7-induced cytotoxicity on NIH3T3 fibroblasts. Conclusion:These results suggest that Na2Cr2O7 showed cytotoxic effect on NIH3T3 fibroblasts by the decrease of cell viavility, and poncirin was effective in the protection of Na2Cr2O7-induced cytotoxicity in these cultures.