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The recent rapid increase in genomic data relatedto many microorganisms and the development of computationaltools to acurately analyze large amounts of data have enabledus to design several kinds of simulation approaches for thecomplex behaviors of cels. Among these aproaches, dFBAequations, and regulatory events, has correctly predicted cellularbehaviors under given environmental conditions. However,until now, dFBA has centered on substrate concentration, celgrowth, and gene on/off, but a detailed hierarchical structureof a regulatory network has not been taken into acount. Theuse of Boolean rules for regulatory events in dFBA haslimited the representation of interactions between specificregulatory proteins and genes and the whole transcriptionalregulation mechanism with environmental change. In this paper,hierarchical structure for a regulatory network with definedfundamental symbols, and introduced a weight between symbolsin order to solve the above problems. Finally, the total controlmechanism of regulatory elements (operons, genes, effectors,etc.) with time was simulated through the linkage of dFBAwith regulatory network modeling. The lac operon, trpoperon, and tna operon in the central metabolic network of E.coli were chosen as the basic models for control patterns. Thesuggested modeling method in this study can be adopted as abasic framework to describe other transcriptional regulations,and provide biologists and engineers with useful informationon transcriptional regulation mechanisms under extracellularenvironmental change.