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This study is to examine the effects of Dhanhak Qigong training which is composed of DoIn exercise, Qigong and DanJeon breathing on the degree of perception of stress and heart rate of workers using daily stress inventory (DSI). The subjects of research were 20 men who are 38 years old and office workers without regular training experience and each 10 were randomly assigned to experimental and control groups. Experimental group had training based on program for 60 min. through three times a week for 10 weeks and two measurements before and after experiment were conducted and then results of measurement were analyzed. As a result of repeated measure two-way ANOVA, mean values of event score, impact score and Impact to Event Score Ratio (I/E ratio) which are sub-factors of DSI showed a significant reduction in experimental group compared to control group. In particular, impact to event score ratio which shows the degree of stress in daily events showed a significant difference in interaction effects of group time. In mean value of heart rate, experimental group showed much more reduction than control group and there was a significant difference in interaction effects of group time. These results suggested that application of Dhanhak Qigong program reduced reaction to daily stress, achieved affirmative physiological effects and then was very helpful for workers' good mental health.