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In the present study, chitosan-gelatin nanoemulsions (CGN) were prepared by Shirasu Porous Glass (SPG) membrane process. The continuous phase (oil phase) consisted of soybean oil and Tween 80, the dispersed phase (water phase) consisted of 0.1% chitosan and 0.1 % gelatin solution. Microscopic observations indicated that the emulsions with mean diameter (Dm) of 300 and 500 nm were reasonably formed. The mean diameter of CGN basically depended upon the SPG membrane pore diameter, meanwhile it was linearly correlated to the amount of Tween 80 with a correlation constant of 0.93-0.99. The CGN prepared with pore diameter (Dp) of 300 and 500 nm, there were great differences in emulsion viscosity when compared to those of control, especially, the CGN prepared with Dp=500 nm was maintained the same level of emulsion viscosity during storage of 9 days.


In the present study, chitosan-gelatin nanoemulsions (CGN) were prepared by Shirasu Porous Glass (SPG) membrane process. The continuous phase (oil phase) consisted of soybean oil and Tween 80, the dispersed phase (water phase) consisted of 0.1% chitosan and 0.1 % gelatin solution. Microscopic observations indicated that the emulsions with mean diameter (Dm) of 300 and 500 nm were reasonably formed. The mean diameter of CGN basically depended upon the SPG membrane pore diameter, meanwhile it was linearly correlated to the amount of Tween 80 with a correlation constant of 0.93-0.99. The CGN prepared with pore diameter (Dp) of 300 and 500 nm, there were great differences in emulsion viscosity when compared to those of control, especially, the CGN prepared with Dp=500 nm was maintained the same level of emulsion viscosity during storage of 9 days.