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Supercritical fluid extraction (SFE) has been used to extract the nomilin from citron seeds. The extraction pressure was increased 100 to 300 bar at the interval of 100 bar, at the temperature 40, 60, 80oC and the extracted time 30, 90, 150 min at CO2 flow rate 2 mL/min. Overall experiments were planned by central composition design and the results were analyzed by response surface methodology to find out the effects of three independent variables such as pressure (X1), temperature (X2) and time (X3) on the yield of nomilin extract (Y). Extraction yields of nomilin were affected by pressure, time and temperature in the decreased order, and the Taylor second order equation was obtained as Y=-650.683283 +11.337258X1-9.569495X2+3.874388X3-0.023381X1 2+0.039897X2 2-0.021832X3 2+0.006699X2X1+0.007193X3X1+ 0.004717X3X2. The optimum condition for the extraction process was obtained as the extraction pressure of 255 bar, and the time of 117 min at the fixed temperature of 46oC.