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본 연구는 기내 배양한 나도풍란의 형태형성 및 GA유사물질 활성에 미치는 생장억제제의 종류 및 농도의 영향에 대해서 조사하였다. 기내 나도풍란의 생장을 억제하기 위한 적절한 농도는 uniconazole과 ancymidol은 0.2mgL-1, paclobutrazol은 0.1mgL-1로 판단되었다. Uniconazole 처리시 잎에서는 GA활성이 거의 나타나지 않았지만 뿌리는 대조구보다 활성이 높게 나타났다. Paclobutrazol 0.3mgL-1 처리에 의해서 표피세포가 조밀하게 배열되었으며, 단위면적당 기공수는 대조구에 비해 1.6배 증가하였으나, 총기공수는 엽면적 감소로 인하여 대조구보다 감소하였다. Paclobutrazol 0.5 mgL-1를 처리한 잎의 종단면을 현미경으로 관찰한 결과 세포층수에는 변화가 없었으나, 대조구에 비해 큐티클층이 두꺼워졌으며 세포의 크기 또한 증가하였다.


This experiment was conducted to clarify the effect of growth retardants on the morphogenesis and activities of GA-like substances in Sedirea japonica in vitro. It was recommended that the desired concentrations of growth reduction in S. japonica were 0.2 mg․L-1 of uniconazole or ancymidol and 0.1 mg․L-1 of paclobutrazol. Uniconazole showed possibility for use not only as a growth retardant but also a growth promoter dew to accelerating effect on both leaf and root growth in the treatment of 0.05-0.1 mg․L-1. Activities of GA-like substances in leaves treated with ancymidol were seldom shown, but in roots, it was higher than the control. Leaf epidermis cells treated with paclobutrazol became more dense than the control. The number of stomata per unit leaf area was increased, but the total number of stomata was decreased compared to the control because of reduction of leaf area. With the microscopic examination of the longitudinal section of leaf, the number of cell layers was the same as the control, but the cell size was increased and the cuticle layer was thickened by paclobutrazol treatment.