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Selection of stress-tolerant ginseng lines in fields is very difficult because it is almostimpossible to control properly the environmental conditions of soil. On the contrary, it canbe studied with ease to search for stress-tolerant ginseng lines through in vitroculturebecause of easy manipulation of stress conditions. This study was conducted for the selectionof ginseng pure lines tolerant to salt stress. Murashige & Skoog(MS) media with 2.5 folds ofKNO3, NH4NO3, MgSO4· 7H2O, KH2PO4, and CaCl2· 2H2O was established for the selectionof ginseng pure lines tolerant to salt stress in vitro. Among 88 ginseng pure lines bred byKorea Ginseng and Tobacco Research Institute, Punggi Hwangsuk, 78093, 82886, 78135,86024 and KG104 lines was tolerant to salt stress. For the stable production of qualityKorean ginseng, genetic tolerance to salt stress is one of important factors since relativelyhigh salt concentrations in the ginseng nursery soil environment of Korea. Ginseng inbredpure lines were tested for their tolerance to salt stress through in vitroculture technique.