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The purified malformin A1 stimulated ethylene production in the primary root segments of maize. Andthis stimulatory effect of malformin A1 was significant in the presence of 0.1 mM IAA. Especially, 10uM malformin A1 increased the ethylene production about 60% of control. Further, malformin A1stimulated the activity of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO), which is thekey enzyme in the ethylene production pathway. And another enzyme in the ethylene productionpathway such as ACC synthase (ACS) was not affected by the malformin A1. Therefore, the increasedethylene production by malformin A1 might be due to the increased in vivo ACO activity in corn roots.


The purified malformin A1 stimulated ethylene production in the primary root segments of maize. And this stimulatory effect of malformin A1 was significant in the presence of 0.1 mM IAA. Especially, 10 uM malformin A1 increased the ethylene production about 60% of control. Further, malformin A1 stimulated the activity of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO), which is the key enzyme in the ethylene production pathway. And another enzyme in the ethylene production pathway such as ACC synthase (ACS) was not affected by the malformin A1. Therefore, the increased ethylene production by malformin A1 might be due to the increased in vivo ACO activity in corn roots.