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Viola ibukiana (Violaceae) distributed in southern part of Korea. In traditional medicine, the herb has been used as an expectorant, a diuretic, and an antiinflammatory for bron- chitis, rheumatism, skin eruptions, and eczema.1,2 Previous phytochemical studies on Viola species have revealed them to be a rich source of cyclotides,3,4 and several flavone glycosides.5,6 Although some common triterpene saponin has been reported, this plant has not been investigated in detail.7 The matrix metalloproteinases (MMPs) are a family of >20 zinc-dependent endoproteinases that are capable of degrading almost all of the components of the extracellular matrix.8 MMPs can be divided into four categories based on substrate preference: collagenases, gelatinases, stromelysins, and membrane-associated matrix metalloproteinases.9 MMPs are necessary for tissue remodeling and the healing cascade under normal physiological condition. The aging process of skin can be divided into intrinsic aging and photoaging. Clinically, naturally aged skin is smooth, pale, and finely wrinkled. In contrast, photoaged skin is coarsely wrinkled.10 Alterations in collagen, the major structural component of skin, have been suggested as a cause of the changes, such as skin wrinkling and loss of elasticity, which are observed in naturally aged and photoaged skin.11 With increasing age, collagen synthesis becomes lower and MMP-1 levels become higher in sun-protected human skin in vivo. UV irradiation induces the synthesis of MMPs in fibroblast cell in vitro and MMP-mediated collagen destruction accounts, in large part, for the connective tissue damage that occurs in photoaging.10 In an ongoing investigation into MMP-1 inhibitory compound from Viola ibukiana, a new active compound was isolated from the EtOAc soluble fraction. In this notes, we report on the constituent of Viola ibukiana, which inhibits UV-induced MMP-1 expression in human skin fibroblasts