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The purpose of this study was to analyze the perception of wayfinding in public interior space. According to this study, a virtual reality simulation test was conducted to examine the effect of wayfinding. Theoretical research was used to explain the psycho-physiological effect of visual perception and case study was carried out to evaluate the physiological response of the subjects. For the physiological evaluation, two simulations with sign and color sample were chosen and the EEG was Used. Data were collected from May 15th. to June 15th. The subjects were 20 students of architecture department and frequency, percentage, ANOVA, T-test, multiple comparisons were used for data analysis.