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This study identified the physiological characteristics of Alnus firma seedlings collected from air pollutedindustrial complex of Yeosu area with inoculation of nitrogen-fixing nodule. A. firma individuals resistantor sensitive to air pollution stress at the study area were selected. Seeds of the individuals were sown ina pot and inoculated with the nodules collected at the same area about 3 weeks after germination. Tocompare the inoculation effects, photosynthesis was measured among the A. firma seedlings under 12inoculation combinations of seed sources and nodule sources. Photosynthetic activities of seedlings fromresistant mother trees were obviously higher than those from sensitive mother tres. In general, theseedlings inoculated with nodule of sensitive trees, showed a worse physiological trend on everycombination. In this study, inoculation effect was less important factor than seed sources to photosyntheticactivities of A. firma. This physiological characteristic seemed to be determined by seed sources rather thannodule sources.