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This study investigated effects of bark acidity, leaf acidity and water soluble sulfur contents by SO2, 1-2 seedling of Prunus yedoensis and Ligustrum obtusifolium were treated 5 level (0. 0.5, 1, 2, 4 ppm) ofSO2. The following results were obtained; In the case of bark and leaf acidities, pH values decreasedas SO2 concentration was high. Prunus yedoensis compare with Ligustrum obtusifolium had highrelative susceptibility because rates of increase in bark and leaf acidities were high according to SO2concentration. Water soluble sulfur contents in the leaves also increased as SO2 concentration washigh. Prunus yedoensis compare with Ligustrum obtusifolium had high relative susceptibility becauseincrease rates of water soluble sulfur contents in the leaves were high according to SO2 concentration.