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This study is an experimental study to investigate the shear behavior of reinforced high-strength concrete deep beams with rectangular openings and presents an equation to determine the shear strength of those beams. Experimental parameters include shear span-to-overall depth ratio(a/h) as well as the width and depth of web opening. Test results indicate that the maximum crack width depended on shear span-to-overall depth ratio rather than size of web opening and the depth of opening had more influence on the shear strength of the beams than the width of opening. Based on Yang & Chung's equation which proposed to predict the shear strength of deep beams, an equation was established by the experimental results, fundamental theory of fracture mechanics, and multiple regression method. Through the comparison of experimental data and analytical results obtained from this and other studies, it was observed that the proposed equation properly estimates the ultimate shear strength of deep beams with rectangular openings.