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The most effective solvent for extract of dye from amur cork tree was methanol. Two protoberberine alkaloids, berberine and palmatine, were isolated from amur cork tree by Prep-TLC, and the developing solvent was Benzene:AcOEt:PrOH:MeOH:EtNH2(8: 4: 2: 0.5: 0.5). Dyes were stable at a high temperature and there wasn't any change of color at pH 3∼9 and with added metal mordants. In adsorption spectra of aqueous solution of berberine and tannin mixture, two isosbestic points(328㎚, 357㎚) were found and the mole fraction of reaction of components was 1:1.