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This experiment was carried out to investigate the primary inoculum of strawberry anthracnose in nursery field. The pathogen, Colletotrichum gloeosporioides was not detected in soil and weeds of nursery field but symptom of anthracnose was developed in mother plants collected from market after incubation in humid chamber. The symptom of anthracnose was expressed in the strawberry plant that reserved for 17 days in field after inoculation by spore suspension but was not observed thereafter. When inoculated leaves were observed by SEM, only appressoria were observed 7 days after inoculation. So, it is guessed that dissemination of Colletotrichum sp. into nursery field will be by contamination of mother plants, and diagnosis by naked eyes may be impossible because symptom will be not developed if environment is to be adequate to penetration and in case of imperfect penetration after germination, the pathogen remains appressorium to achieve penetration.