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In this study, antibacterial activity on the chicken feed supplemented with different concentrations of oriental herbal medicine feed additives (OHMFA) extract was tested for some organisms and their preventive effects on the colonization of Salmonella enteritidis (SE) in broiler chickens were examined. The growth of Bacillus cereus, Listeria monocytogenes and Staphylococcus aureus was inhibited on the feed of 0.25 % OHMFA and Salmonella spp. and Escherichia coli O157:H7 were inhibited on the feed containing 2.0 % level. The broiler chickens fed a forage additived 1.0 % level of OHMFA after inoculation of a 107 CFU/ml of SE were increased in weight than the chicken fed forage without OHMFA. The incidence of SE in liver, spleen and cecum of the chickens fed forage with OHMFA was decreased on the 7th days of postinfection and SE was not isolated from the organs of the chickens after the 14th days of postinfection. Serum antibody titers of the chickens were lowered than the control group.