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The employees in information system organization are highly exposed to stress. However, there is little theoretical or empirical study on the effects of job stress on IS organizations. A major reason is because few researchers consider consequences of job stress. The major purpose of this study is to verify negative effects of job stress for IS organization. This study have three purposes: to investigate the relationship between job stressors and perceived job stress; to understand the effect of perceived job stress and job attitude - job satisfaction, job involvement; to examine the role of moderators such as social support and personality in the relationship between job stressors and perceived job stress in information system organizations. The results of this study suggest that job stressors such as role overload and role ambiguity are significantly related to perceived job stress, the higher the perceived job stress is the lower the job attitudes such as job satisfaction and job involvement, and the employees' personality has moderating effects between the job stressor and perceived job stress.