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Background and Objectives:This study was conducted to investigate the healing effect of the low-level laser iradiation on wound healing in vivo using DPSS laser (532 nm) and Diode laser (660 nm). Materials and Method:Each mouse received dorsal, full-thicknes round incision (= 2 cm) and daily laser irradiation (4 J/cm2) was done before sacrifice. On sacrifice at 3, 7, 10 days, the wound was excised, then wound closure and histologic stages were measured, and standardized. Results:The percentages of wound closure in DPSS laser, Diode laser, control were 33.2± 2.4, 34.2± 3.5, 24.0± 2.7 at day 3, 64.8± 3.5, 72.2± 2.8, 42.8± 5.0 at day 7 and 82.2± 7.9, 87.2± 3.7, 71.4± 4.0 at day 10, respectively, with p<0.05. Histological evaluation showed that laser irradiation enhanced wound epithelialization, celular content deposition, granulation tissue formation, colagen deposition and neovascularization in the laser-treated wounds as compared to the control group. Conclusion:Low-level laser irradiation at 532 nm and 660 nm significantly enhanced cutaneous wound healing effect in the wounded mouse model. Further investigation of the mechanism of low-level laser therapy in primary wound healing is waranted. (Korean J Otolaryngol 203 ; 46 :851-5)