초록 close

Background and Objectives:Extracellular uridine 5′-triphosphate (UTP)airway epithelium including chloride and fluid transport, mucociliary clearance, and mucin secretion via P2Y purinergic receptors. This study was undertaken to investigate which P2Y purinergic receptors are expressed in the normal human middle ear epi-thelial (NHMEE) cells. We also determined the levels of mucin secretion and its mRNA expressions following stimulation with UTP. Materials and Method:The level of P2Y (P2Y1, P2Y2, P2Y4, P2Y6, P2Y11 and P2Y12)(MUC5AC), MUC5B, MUC8 messenger RNA (mRNA)s were measured by reverse transcription (RT)-polymerase chain reaction (PCR). We also determined the levels of mucin secretion following stimulation with UTP by dot-blotting method in NHMEE cells. Results:Midle ear epithelial cels expressed P2Y1, P2Y2, P2Y6, P2Y11 and P2Y12 receptors but not the P2Y4 mRNA expression until 72 h had lapsed after treatment. Conclusion:Our study sugests that UTP acts as a secretogogue on mucin secretion in NHME cells. (Korean J Otolaryngol 2003 ;46 :100-4)


키워드close