초록 close

Background and Objectives:Aural cholesteatoma is a destructive disease of the middle ear caused by progresive expansion of keratinizing squamous epithelium. Previous studies reported that apoptosis of keratinizing squamous epithelium was incre-of apoptosis using expresion of bcl-2, p53 and bax in various sites of experimental cholesteatomas. We also investigated the degree of apoptosis in different clinical stages of cholesteatoma. Materials and Method:Using canal ligation cholesteatoma in mongolian gerbil, we performed imunohistochemical stainings of bcl-2, p53 and bax. Labeling indices of bcl-2 and p53 expre-ssoin and subjective grading of bax expression were measured and analyzed according to the site and clinical stage of chole-steatoma. Results:1) Expression of bcl-2 and p53 were significantly increased at lateral external auditory canal compared with those at the tympanic membrane. 2) Expression of bax of cholesteatoma was significantly increased compared with that of the normal external auditory canal. 3) Expresion of bcl-2 and bax were significantly increased in the stage I and expresion of p53 was increased in the stage III of cholesteatoma. Conclusion:Changes of bcl-2, p53 and bax expresion in experimental chole-according to the diferent sites and diferent clinical stages of cholesteatoma. (Korean J Otolaryngol 2003 ;46 :8-15)


키워드close