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This experiment study aimed to identify the continuous genetic variation caused by benzyladenine (BA) treatment in strawberry tissue culture. The ‘Goha’ cultivar was used and treated with different concentrations of BA (0.0, 0.5, 1.0, 2.0 mg・L-1). Morphological and genetic variation tests were performed, and genetic continuity tests were performed for three years. The morphological variation induced by BA was distinctively high (10.5 ~ 20.0%) and the genetic variation was 7.0 ~ 15.0%, 1.8 ~ 10.0%, and 5.0% in the first, second, and third year of cultivation, respectively. The rate of genetic variation decreased with increasing cultivation years. In addition, genetic variation caused by BA 1.0 mg・L-1 and BA 2.0 mg・L-1 occurred in the first and second years of cultivation, whereas only BA 2.0 mg・L-1 caused genetic variation in the third year of cultivation. Therefore, a concentration of less than 1.0 mg・L-1 BA was used for the propagation of strawberry tissue culture plants, and it was necessary to identify their variation.