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This study was carried out to examine the effects of bis (tribytyltin)oxide (TBT), endocrine disruptingParalichthys olivaceus. Oliver Flounders were exposed to sublethal concentration of TBT (0, 1.67, 3.20, 6.30and 12.50g/L) during 6 weeks. Survival rate was decreased in a concentration and exposure period-dependent way and suddenly the reduction of more than 20% occurred at TBT concentration greater than 3.20g/L after exposure 6 weeks. Growth rate and feed efficiency significantly decreased at concentration greaterthan 3.20g/L. Oxygen consumption rate was also decreased in a concentration-dependent way andsignificantly decreased to 17, 48 and 67% than that of the control at the TBT concentration of 3.20, 6.32 and12.50g/L, respectively. This study revealed that high TBT concentration (≥3.20g/L) reduced growth andoxygen consumption rates of the juvenile oliver flounder suggesting potential influence on the natural mortalityof Paralichthys olivaceusin the coastal areas.