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A rapid, inexpensive enzymatic method is proposed for indirect water quality testing in terms of heavy metaltoxicity. The activity of glucose-6-phosphate dehydrogenase was applied for heavy metal toxicity test as aneffective criterion in water quality. The toxicity of Pb(lead) and Cd(cadmium) for water flea, Moina macrocopa, were evaluated for 2~ 8 dayswith variables of mobilization ability. And the reproduction impairment of Moina macrocopawere investigatedas the parameter of chronic toxicity test for Pb and Cd. As a result, the EC50 for immobilization of Moinamacrocopawere Pb and Cd were 1.6749 and 0.4683, respectively. The values of reproductive impairment toMoina macrocopafor Pb and Cd were 9.5938 and 8.3264 in EC50A significant alteration of G6PDH (Glucose-6-phosphate dehydrogenase) activity of Moina macrocopawas observed when Cd and Pb were treated in media. The results obtained indicate that G6PDH activity ofMoina macrocopacan be used as an indicative parameter in aquatic toxicity tests for heavy metals.