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Purpose of this research was to focused on change of Insulin counter-regulatory hormone and body composition after 3wk, 6wk, 9wk walking.Subjects of the research were 40-50yrs NIDDM women patients who never participate exercise. The measured data were analyzed by SPSS for windows 10.0 and one-way repeated ANOVA.The results of this were as follows :Weight, Fat mass, Percent fat, Lean body mass, BMI of body composition were decreased as increase of exercise period. No difference appear on Weight, Fat mass, Percent fat, Lean body mass, BMI of body composition under one- way repeated ANOVA analysis.Concentrations of Glucagon and Growth Hormone based on change of Insulin counter-regulatory hormone were decreased as increased of exercise period. However, Cortisol was increased as exercise period was increased.One-way repeated ANOVA analysis for change of Insulin counter-regulatory hormone presented difference on Glucagon.The research found that Walking was very effective in control of NIDDM and additional experiments for design more effective exercise program were required.1) 대구보건대학702-722 대구 북구 태전동 산7번지1. Daegu Health CollegeSan#7 Taegjundong Bukgu Daegu 702-722 Korea2) 대구 광역시 북구 보건소702-053 대구 북구 침산3동 521-2 2. BukGu Health Center, Daegu city521-2 Chimsan 3-Dong Buk-Gu Daegu 702-053 Korea