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FISH(Fluorescence in situ Hybridization) technique was applied to the several nitrifiers in the bulk solution and on the Cilium media to promote nitrification process.It was confirmed by the results of Nso190 and NEU image analysis that ammonia oxidizer forms 'cluster' when they appears in both bulk and media. Another significant fact was that lots of microbial flocs were observed on the surface of the Cilium media substratum instead of biofilm. And from the results of NEU, it was known that Nitrosomonas europaea, Nitrosomonas eutopha and Nitrococcus mobilis or one or two of these three were the dominant ammonia oxidizers.The fact that no Nitrobacter sp. was detected with NIT3 probe means naturally that nitrite oxidizer in the nitrification reactor was not composed of Nitrobacter sp. but with other nitrite oxidizers like Nitrospira sp. etc.. In order to detect these nitrite oxidizers, suitable probes must be tested.