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A water quality analysis of on the stream as well as local stream has been depending on the most chemical methods. But, if the measuring methods of both chemical and ecological accomplished, the variation of water quality on the constant duration can be measure during short time as well as the stream quality can predict to more continues and it can be reliability The purpose of this study were to measure the Biological Index(Diversity Index, Dominance Index, Evenness Index, Richness Index) as indicators of environmental impact assessment method. The plants and the creature living in watershed of Anyang stream and the tens branches of it were investigated. These results were divided to the three steps based on the area of water by self-purification step as follow; the sevens stream and mainstream were stronger corrosion waters, one stream of beta-middle corrosion waters and twos stream of alpha-middle corrosion waters. Through those, the index of environmental impact assessment could be found, and it was could be presented Predominance, Diversity and creation of a species