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In this study, to minimize the uncertainty of model parameters in QUAL2E model, we have optimized the parameter using evolution algorithm, and applied this to the Tamjin River basin. we have confirmed a superiority in the applicability of parameter estimation of QUAL2E by evolution algorithm, compared with trial and error method. Also, BOD concentrations of the Tamjin River are estimated by QUAL2E model for years of 2007 and 2011.