ABSTRACT

Background: A large number of studies have been focused on bacterial growth but limited number of literature has been reported regarding modification of fungal growth. Objective: This study aims to investigate effects of low alternating current on Microsporum (M.) canis and Trichophyton (T.) tonsurans growth. Methods: Inoculums of M. canis and T. tonsurans were applied to twenty-four PDACT (potato dextrose agar-corn meal-Tween 80) plates with a sterile spreader. Petri dishes were allocated into 8 groups according to the fungi species and the amperage delivered to these dishes. Group A, B, C and D were M. canis group and E, F, G, H were T. tonsurans group. The given amperage of electric current was 0.5 μA in group A and E, 2 μA in B and F, 4 μA in C and G. No electric current was given in group D and H. Results: In groups A, B, and C the average time elapsed for colony appearances were 42 hours, 43.17 hours, and 40.5 hours respectively. The average time elapsed in the control group D was 88.67 hours. In groups E, F, and G the average time elapsed for colony appearances were 63.67 hours, 61.83 hours, and 64.17 hours respectively. The average time elapsed in the control group H was 90.60 hours. Conclusion: With electric current, faster fungal growth was observed in the amperage range used in this study. Based on these results, we hypothesized that microcurrent helps the fungal growth.

KEYWORD

Electric stimulus, Fungal growth, Microcurrent, Microsporum canis, Trichophyton tonsurans

REFERENCES(14)open

  1. [jounal] Kalinowski DP, Edsberg LE, Hewson RA, Johnson RH, Brogan MS / 2004 / Low-voltage direct current as a fungicidal agent for treating onychomycosis / J Am Podiatr Med Assoc 94 : 565 ~ 572

  2. [jounal] Kwon DR, Kwon H, Lee WR, Park J / 2016 / Investigating effects of nano- to micro-ampere alternating current stimulation on Trichophyton rubrum growth / Ann Dermatol 28 : 575 ~ 578

  3. [jounal] Lee WJ, Song CH, Lee SJ, Kim DW, Jun JB, Bang YJ / 2012 / Decreasing prevalence of Microsporum canis infection in Korea: through analysis of 944 cases (1993-2009) and review of our previous data (1975-1992) / Mycopathologia 173 : 235 ~ 239

  4. [jounal] Brajac I, Stojnic-Sosa L, Prpic L, Loncarek K, Gruber F / 2004 / The epidemiology of Microsporum canis infections in Rijeka area, Croatia / Mycoses 47 : 222 ~ 226

  5. [jounal] Flari ME, Graser Y, Presber W, Tietz HJ / 2000 / An epidemic of tinea corporis caused by Trichophyton tonsurans among children in Germany / Mycoses 43 : 191 ~ 196

  6. [jounal] Leeming JG, Elliott TS / 1995 / The emergence of T. tonsurans tinea capitis in Birmingham, U.K / Br J Dermatol 133 : 929 ~ 931

  7. [jounal] Cheng N, Van Hoof H, Bockx E, Hoogmartens MJ, Mulier JC, De Dijcker FJ, et al / 1982 / The effects of electric currents on ATP generation, protein synthesis, and membrane transport of rat skin / Clin Orthop Relat Res 171 : 264 ~ 272

  8. [jounal] Mitchell P / 1966 / Chemiosmotic coupling in oxidative and photosynthetic phosphorylation / Biol Rev Camb Philos Soc 41 : 445 ~ 502

  9. [jounal] Mitchell P / 1976 / Vectorial chemistry and the molecular mechanism of chemiosmotic coupling: Power transmission by proticity / Biochem Soc Trnas 4 : 399 ~ 430

  10. [jounal] Kaziro Y / 1978 / The role of guanosine-5'-triphosphate in polypeptide chain elongation / Biochim Biophys Acta 505 : 95 ~ 127

  11. [jounal] Keller EB, Zamecnik PC / 1956 / The effects of guanosine diphosphate and triphosphate on the incorporation of labeled amino acids into proteins / J Biol Chem 221 : 45 ~ 59

  12. [jounal] Hubel KA / 1978 / The effects of electrical field stimulation and tetrodotoxin on ion transport by isolated rabbit ileum / J Clin Invest 62 : 1039 ~ 1047

  13. [jounal] Gow NAR / 1989 / Relationship between growth and the electrical current of fungal hyphae / Biol Bull 176 : 31 ~ 35

  14. [jounal] Bozoky L, Kiszely G, Hoffman TA, Ladik J / 1963 / Effect of electrostatic fields on cell mitosis / Nature 199 : 1306