ABSTRACT

To clarify the cytotoxicity induced by lead acetate (LA) and the protective effect of Elscholtziae splendense (ES) extract on the LA-induced cytotoxicity, cultured glioma cells (C6 glioma) were treated with or without LA or ES extract. In this study, LA significantly decreased the cell viability. While, vitamin E protected LA-induced cytotoxicity by the increase in cell viability which was decreased by LA. In the protective effect of ES extract on LA-induced cytotoxicity, ES extract prevented LA-induced cytotoxicity by a significant increase in cell viability, and showed the DPPH-radical scavenging activity, superoxide dismutase (SOD)-like activity and inhibitory activity of lactate dehydrogenase (LDH). From these results, it is suggested that the cytotoxicity of LA is involved in oxidative stress, and ES extract prevented LA-induced cytotoxicity effectively. And also, it showed the antioxidative effects such as DPPH-radical scavenging activity, SODlike activity and inhibitory activity of LDH. Conclusively, the plant extract like ES may be a putative resources for the beauty by the diminution or blocking the cytotoxicity or oxidative stress mediated by heavy metals or chemicals which are components of hair dye or cosmetics.

KEYWORD

Elscholtziae splendense extract, Glial cell, Hair dye, Oxidative stress

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