ABSTRACT

The purpose of this study was to investigate the effect of Gardeniae fructus water extract (GFWE) and Saururus chinensis water extract (SCWE) on the antioxidative and collagen synthetic abilities. The contents of total polyphenol in GFWE and SCWE were 66.1 mg/g and 53.5 mg/g, respectively. The contents of total flavonoid in GFWE and SCWE were 34.5mg/g and 28.7 mg/g, respectively. Electron donating abilities of GFWE, SCWE and BHT (positive control) showed a dose response relationship and they were 24.6%, 21.3% and 82.5% at 1,000 ppm, respectively. GFWE and SCWE have potent free radical scavenging activity even though showing lower value than that of BHT. In human dermal fibroblasts, cell growth activities of GFWE and SCWE were more than 90% in all the measured concentrations. Thus, the maximum permissible levels of GFWE and SCWE in human dermal fibroblasts were confirmed to be more than 200 ppm. TGF-β_1 (5ng/mL) increased type-1 procollagen protein production in cultured human dermal fibroblasts by 127%. GFWE and SCWE increased the procollagen protein production by 34.5% and 33.9%, respectively, at the concentration of 100 ppm.

KEYWORD

Antioxidative activity, Gardeniae fructus, Procollagen, Saururus chinensis

REFERENCES(39)open

  1. [jounal] 강성례 / 2009 / 삼백초 열수추출물의 피부주름 억제효과 / 한국미용학회지 15 (4) : 1389 ~ 1398

  2. [jounal] 고진애 / 2010 / In vitro에서 음양곽 열수추출물의 항산화능 및 멜라닌 생성 촉진능 / 대한미용학회지 6 (3) : 279 ~ 286

  3. [jounal] 김정균 / 2003 / 천연한약재(목통, 삼릉, 치자) 추출물의 항산화효과 및 항균활성효과 / 농업생명과학연 구 37 (4) : 69 ~ 75

  4. [thesis] 김필순 / 2009 / 백지 에탄올 추출물의 미백효과 및 멜라닌합성 저해 기 전 연구

  5. [jounal] 양웅모 / 2006 / 연잎 에탄올 추출물이 피부 주름에 미치는 영향 / 대한한의학 방제학회지 14 (2) : 67 ~ 75

  6. [jounal] 이웅룡 / 2007 / 플라보노이드의 세포 신호전달 조절 / 대예방학회지 12 (3) : 163 ~ 173

  7. [jounal] 형순희 / 2009 / Hairless 마우스에서 치자 열수추출물의 피부 주름형성 억제효과 / 대한피부미용학회지 7 (2) : 123 ~ 136

  8. [jounal] Abdelwahed A / 2007 / Study of Antimutagenic and Antioxidant Activities of Gallic Acid and 1,2,3,4,6-Pentagalloylglucose from Pistacia lentiscus Confirmation by Microarray Expression Profiling / Chem-Biol Interact 165 (1) : 1 ~ 13

  9. [jounal] Abdullaev FI / 1994 / Inhibitory effect of crocetin on intracellular nucleic acid and protein synthesis in malignant cells / Toxicol Letters 70 (2) : 243 ~ 251

  10. [book] A.O.A.C / 1995 / Official methods of analysis (12th) / A.O.A.C : 127 ~ 130

  11. [book] Bateman JF / 1996 / Collagen superfamily extracellular matrix / Harwood : 22 ~ 67

  12. [jounal] Blois MS / 1958 / Antioxidant determination by the use of a stable free radical / Nature 181 : 1199 ~ 1200

  13. [jounal] Carmichael J / 1987 / Evaluation of a tetrazolium-based semiautomated colorimetri assay: assessment of chemosensitivity testing / Cancer Res 47 (4) : 936 ~ 942

  14. [jounal] Chiba K / 2003 / Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin / Skin Pharm Appl Skin Physiol 16 : 242 ~ 251

  15. [jounal] 최용민 / 2003 / 국내 시판되는 일부 다류 제품의 항산화 효과 / 한국식품영양과학회지 32 (5) : 723 ~ 727

  16. [jounal] Duell EA / 1996 / Extraction of human epidermis treated with retinol yields retro-retinoid in addition to free retinol and retinyl esters / J Invest Dermatol 107 (2) : 278 ~ 282

  17. [jounal] Folin O / 1912 / On phosphotungstic-phosphomolybdic compounds as color reagents / J Biol Chem 12 : 239 ~ 243

  18. [jounal] Halliday GM / 2005 / Inflammation, gene mutation and photoimmunosuppression in response to UVR-induced oxidative damage contributes to photocarcinogenesis / Mut Res 571 (1) : 107 ~ 120

  19. [jounal] Han YN / 1994 / Antioxidant components / Kor J Pharmacog 25 : 226 ~ 232

  20. [jounal] Jasna B / 2007 / Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey / Food Chem 105 : 822 ~ 828

  21. [jounal] Jenkins G / 2002 / Molecular mechanisms of skin aging / Mech Ageing and Develop 123 (7) : 801 ~ 810

  22. [jounal] Jenner P / 1996 / Oxidative stress and the pathogenesis of Parkinson’s disease / Neurology 47 (6) : 161 ~ 170

  23. [jounal] 강명화 / 2007 / 부위별 식용백합 추출물의 항산화 및 항균효과 / 한국식품과학회지 39 (4) : 452 ~ 457

  24. [jounal] Kang S / 2005 / transcription factors nuclear factor-kB and / Am J Pathol 166 (6) : 1691 ~ 1699

  25. [jounal] 이상옥 / 2005 / 차가버섯의 온도단계별 물추출물의 항산화성 비교 / 한국식품영양과학회지 34 (2) : 139 ~ 147

  26. [jounal] Leighton T / 1992 / Molecular characterization of quercetin and quercetin glycosides in allium vegetable: their effects on malignant cell transformation / Am Chem Soc Sym Series 507 : 220 ~ 238

  27. [jounal] Lowry OH / 1951 / Protein measurement with the folin phenol reagent / J Biol Chem 193 (1) : 265 ~ 275

  28. [jounal] Mahadik SP / 1996 / Free radical pathology and antioxidant defense in schizophrenia: a review / Schizophrenia Res 19 : 1 ~ 17

  29. [jounal] Parfitt AM / 1987 / Pro-collagen type I carboxy-terminal extension peptide in serum as a marker of collagen biosynthesis in bone. Correlation with iliac bone formation rates and comparison with total alkaline phosphatase / J Bone Min Res 2 (5) : 427 ~ 436

  30. [jounal] Rice-Evans C / 2001 / Flavonoid antioxidants / Curr Med Chem 8 : 797 ~ 807

  31. [jounal] Saurat JH / 1999 / Topical natural retinoids: the ‘proligand-nonligand’ concept / Dermatology 199 (1) : 1 ~ 2

  32. [jounal] Son ED / 2007 / Alphaketoglutarate stimulates procollagen production in cultured human dermal fibroblasts, and decreases UVB-induced wrinkle formation following topical application on the dorsal skin of hairless mice / Biol Pharm Bull 30 (8) : 1395 ~ 1399

  33. [jounal] Talwar HS / 1995 / Reduced type I, type III procollagens in photodamaged adult human skin / J Invest Dermatol 105 (2) : 285 ~ 290

  34. [jounal] Velioglu YS / 1998 / Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products / J Agr Food Chem 46 : 4113 ~ 4117

  35. [jounal] Waldrop M / 1980 / Firm takes new approach to food additives / Chem Engin News 55 : 22 ~ 27

  36. [jounal] Wang M / 1998 / Antioxidative phenolic compound from sage (Salvia officinalis) / J Agr Food Chem 46 : 4869 ~ 4873

  37. [jounal] Wlaschek M / 2001 / Solar UV irradiation and dermal photoaging / J Photochem Photobiol B Biol 63 : 41 ~ 51

  38. [jounal] Wyss R / 1995 / Chromatographic and electrophoretic analysis of biomedically important retinoids / J Chrom B: Biom Sci App 671 : 381 ~ 425

  39. [jounal] Yim CK / 1999 / Isolation of 3,4-Dihydroxybenzoic acid, which exhibits antimicrobial activity, from fruits of Gardenia jasminoides Ellis / J Food Sci Technol 31 (5) : 1386 ~ 1391